{"id":34,"date":"2014-10-28T15:11:10","date_gmt":"2014-10-28T15:11:10","guid":{"rendered":"https:\/\/protsci-us2b.univ-nantes.fr\/imut\/?page_id=34"},"modified":"2023-05-03T08:44:27","modified_gmt":"2023-05-03T08:44:27","slug":"imut-saturate","status":"publish","type":"page","link":"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/?page_id=34","title":{"rendered":"iMutate saturate"},"content":{"rendered":"<h2>Site saturation mutagenesis (SSM)<\/h2>\n<p>This script performs a <strong>site saturation mutagenesis<\/strong> (SSM) on <strong>all positions<\/strong> of a protein sequence. For each position, the script generates 19 other sequences with an amino acid different from the wild sequence at this position. The total number of sequences is n*19 with n that is the length of the sequence.<\/p>\n<p>If you want to saturate only one or a few sites, use the general <a href=\"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/?page_id=43\" title=\"Combinatorial site saturation mutagenesis\">Saturate\/combine<\/a> tool.<br \/>\n<a href=\"https:\/\/i0.wp.com\/protsci-us2b.univ-nantes.fr\/imutate\/wp-content\/uploads\/2014\/10\/saturate.png?ssl=1\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/i0.wp.com\/protsci-us2b.univ-nantes.fr\/imutate\/wp-content\/uploads\/2014\/10\/saturate.png?resize=827%2C556&#038;ssl=1\" alt=\"saturate\" class=\"alignnone size-full wp-image-90\" width=\"827\" height=\"556\" srcset=\"https:\/\/i0.wp.com\/protsci-us2b.univ-nantes.fr\/imutate\/wp-content\/uploads\/2014\/10\/saturate.png?w=827&amp;ssl=1 827w, https:\/\/i0.wp.com\/protsci-us2b.univ-nantes.fr\/imutate\/wp-content\/uploads\/2014\/10\/saturate.png?resize=300%2C201&amp;ssl=1 300w\" sizes=\"auto, (max-width: 827px) 100vw, 827px\" data-recalc-dims=\"1\" \/><\/a><\/p>\n<table>\n<tbody><\/tbody>\n<\/table>\n<p>Write or upload your sequence.<\/p>\n<form action=\"https:\/\/protsci-us2b.univ-nantes.fr\/cgi-bin\/imut\/imut_saturatebis.cgi\" enctype=\"multipart\/form-data\" method=\"post\" target=\"_top\"><textarea cols=\"50\" rows=\"10\" name=\"sequencebox\"><\/textarea><\/p>\n<p><input maxlength=\"80\" name=\"sequenceupload\" size=\"50\" type=\"file\"><br \/>\n<input name=\"Submit\" type=\"submit\" value=\"Submit\"><br \/>\n<input name=\".reset\" type=\"reset\"><br \/>\n<\/form>\n","protected":false},"excerpt":{"rendered":"<p>Site saturation mutagenesis (SSM) This script performs a site saturation mutagenesis (SSM) on all positions of a protein sequence. For each position, the script generates 19 other sequences with an amino acid different from the wild sequence at this position. The total number of sequences is n*19 with n that is the length of the &hellip; <\/p>\n<p class=\"link-more\"><a href=\"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/?page_id=34\" class=\"more-link\">Read more<span class=\"screen-reader-text\"> &#8220;iMutate saturate&#8221;<\/span><\/a><\/p>\n","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-34","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/index.php?rest_route=\/wp\/v2\/pages\/34","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=34"}],"version-history":[{"count":11,"href":"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/index.php?rest_route=\/wp\/v2\/pages\/34\/revisions"}],"predecessor-version":[{"id":241,"href":"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/index.php?rest_route=\/wp\/v2\/pages\/34\/revisions\/241"}],"wp:attachment":[{"href":"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=34"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}