{"id":49,"date":"2014-10-28T16:39:07","date_gmt":"2014-10-28T16:39:07","guid":{"rendered":"https:\/\/protsci-us2b.univ-nantes.fr\/imut\/?page_id=49"},"modified":"2023-05-05T12:30:51","modified_gmt":"2023-05-05T12:30:51","slug":"imut-chimera","status":"publish","type":"page","link":"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/?page_id=49","title":{"rendered":"iMutate chimera"},"content":{"rendered":"<h2>Gene shuffling mutagenesis<\/h2>\n<p>This script splits up two <strong>parent<\/strong> protein sequences into a given number of fragments. Next it shuffles the fragments between the two parent proteins. The tool keeps the fragments in the same order as in the parents.<\/p>\n<h3>Example<\/h3>\n<p>In order to undertand the tool, see the following example:<\/p>\n<ul>\n<li>Parent protein A (green) that will be cut before positions 120 and 380 for generating three fragments<\/li>\n<li>Parent protein B  (blue), that will be cut before positions 120 and 370 for generating three fragments<\/li>\n<\/ul>\n<p><a href=\"https:\/\/i0.wp.com\/protsci-us2b.univ-nantes.fr\/imutate\/wp-content\/uploads\/2014\/10\/chimera2.png?ssl=1\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/i0.wp.com\/protsci-us2b.univ-nantes.fr\/imutate\/wp-content\/uploads\/2014\/10\/chimera2.png?resize=475%2C339&#038;ssl=1\" alt=\"chimera\" class=\"alignnone size-full wp-image-60\" width=\"475\" height=\"339\" srcset=\"https:\/\/i0.wp.com\/protsci-us2b.univ-nantes.fr\/imutate\/wp-content\/uploads\/2014\/10\/chimera2.png?w=475&amp;ssl=1 475w, https:\/\/i0.wp.com\/protsci-us2b.univ-nantes.fr\/imutate\/wp-content\/uploads\/2014\/10\/chimera2.png?resize=300%2C214&amp;ssl=1 300w\" sizes=\"auto, (max-width: 475px) 100vw, 475px\" data-recalc-dims=\"1\" \/><\/a><\/p>\n<form action=\"https:\/\/protsci-us2b.univ-nantes.fr\/cgi-bin\/imut\/imut_chimeric.cgi\" enctype=\"multipart\/form-data\" method=\"post\" target=\"_top\">\n<table col=\"1\" width=\"50%\">\n<tbody>\n<tr>\n<td>Positions for cuts for protein 1 (one per line)<br \/>\n<textarea cols=\"10\" rows=\"5\" name=\"cuts\"><\/textarea><\/td>\n<td>Positions for cuts for protein 2 (one per line)<br \/>\n<textarea cols=\"10\" rows=\"5\" name=\"cuts2\"><\/textarea><\/td>\n<\/tr>\n<tr>\n<td>or upload cut positions for protein 1<br \/>\n<input maxlength=\"80\" name=\"cuts_up\" size=\"20\" type=\"file\"><\/td>\n<td>or upload cut positions for protein 2<br \/>\n<input maxlength=\"80\" name=\"cuts_up2\" size=\"20\" type=\"file\"><\/td>\n<\/tr>\n<tr>\n<td>Write or upload your sequence 1<br \/>\n<textarea cols=\"50\" rows=\"10\" name=\"sequencebox\"><\/textarea><\/td>\n<td>Write or upload your sequence 2<br \/>\n<textarea cols=\"50\" rows=\"10\" name=\"sequencebox2\"><\/textarea><\/td>\n<\/tr>\n<tr>\n<td><input maxlength=\"80\" name=\"sequenceupload\" size=\"50\" type=\"file\"><\/td>\n<td><input maxlength=\"80\" name=\"sequenceupload2\" size=\"50\" type=\"file\"><\/td>\n<\/tr>\n<tr>\n<td><\/td>\n<td><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><input name=\"Submit\" type=\"submit\" value=\"Submit\"><br \/>\n<input name=\".reset\" type=\"reset\"><br \/>\n<\/form>\n","protected":false},"excerpt":{"rendered":"<p>Gene shuffling mutagenesis This script splits up two parent protein sequences into a given number of fragments. Next it shuffles the fragments between the two parent proteins. The tool keeps the fragments in the same order as in the parents. Example In order to undertand the tool, see the following example: Parent protein A (green) &hellip; <\/p>\n<p class=\"link-more\"><a href=\"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/?page_id=49\" class=\"more-link\">Read more<span class=\"screen-reader-text\"> &#8220;iMutate chimera&#8221;<\/span><\/a><\/p>\n","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-49","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/index.php?rest_route=\/wp\/v2\/pages\/49","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=49"}],"version-history":[{"count":12,"href":"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/index.php?rest_route=\/wp\/v2\/pages\/49\/revisions"}],"predecessor-version":[{"id":247,"href":"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/index.php?rest_route=\/wp\/v2\/pages\/49\/revisions\/247"}],"wp:attachment":[{"href":"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=49"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}