{"id":6,"date":"2014-10-07T11:36:20","date_gmt":"2014-10-07T11:36:20","guid":{"rendered":"https:\/\/protsci-us2b.univ-nantes.fr\/imut\/?page_id=6"},"modified":"2026-02-16T14:48:37","modified_gmt":"2026-02-16T14:48:37","slug":"imut-simplex","status":"publish","type":"page","link":"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/?page_id=6","title":{"rendered":"iMutate Simple"},"content":{"rendered":"<h2>Single point mutagenesis<\/h2>\n<p>This script introduces single point mutation into a target sequence.<br \/>\nYou can give a list of single point mutation to process.<\/p>\n<p><a href=\"https:\/\/i0.wp.com\/protsci-us2b.univ-nantes.fr\/imutate\/wp-content\/uploads\/2014\/10\/simplex31.png?ssl=1\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/i0.wp.com\/protsci-us2b.univ-nantes.fr\/imutate\/wp-content\/uploads\/2014\/10\/simplex31.png?resize=760%2C328&#038;ssl=1\" alt=\"simplex3\" class=\"alignnone size-full wp-image-73\" width=\"760\" height=\"328\" srcset=\"https:\/\/i0.wp.com\/protsci-us2b.univ-nantes.fr\/imutate\/wp-content\/uploads\/2014\/10\/simplex31.png?w=760&amp;ssl=1 760w, https:\/\/i0.wp.com\/protsci-us2b.univ-nantes.fr\/imutate\/wp-content\/uploads\/2014\/10\/simplex31.png?resize=300%2C129&amp;ssl=1 300w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" data-recalc-dims=\"1\" \/><\/a><\/p>\n<form action=\"https:\/\/protsci-us2b.univ-nantes.fr\/cgi-bin\/imut\/imut_simplex.cgi\" enctype=\"multipart\/form-data\" method=\"post\" target=\"_top\">\n<table style=\"text-align: left; width: 100%; overflow: auto;\" cellspacing=\"2\" cellpadding=\"2\" border=\"0\">\n<tbody>\n<tr>\n<td><strong>Mutation list<\/strong><\/td>\n<td><strong>Wild-type sequence<\/strong><\/td>\n<\/tr>\n<tr>\n<td>Write your mutation list (one per line):<br \/>\n<textarea cols=\"20\" rows=\"5\" name=\"Listmutation\"><\/textarea><br \/>\nExample:<br \/>\nP1T<br \/>\nC5F<\/td>\n<td>Write your WT sequence (in fast format):<br \/>\n<textarea cols=\"50\" rows=\"10\" name=\"sequencebox\"><\/textarea><\/td>\n<\/tr>\n<tr>\n<td>or upload your list<br \/>\n<input maxlength=\"80\" name=\"Listmutationup\" size=\"30\" type=\"file\"><\/td>\n<td>or upload your sequence<br \/>\n<input maxlength=\"80\" name=\"sequenceupload\" size=\"30\" type=\"file\" accept=\".fasta\"><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><input name=\"Submit\" type=\"submit\" value=\"Submit\"><br \/>\n<input name=\".reset\" type=\"reset\"><\/p>\n<\/form>\n","protected":false},"excerpt":{"rendered":"<p>Single point mutagenesis This script introduces single point mutation into a target sequence. You can give a list of single point mutation to process. Mutation list Wild-type sequence Write your mutation list (one per line): Example: P1T C5F Write your WT sequence (in fast format): or upload your list or upload your sequence<\/p>\n","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-6","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/index.php?rest_route=\/wp\/v2\/pages\/6","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=6"}],"version-history":[{"count":25,"href":"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/index.php?rest_route=\/wp\/v2\/pages\/6\/revisions"}],"predecessor-version":[{"id":258,"href":"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/index.php?rest_route=\/wp\/v2\/pages\/6\/revisions\/258"}],"wp:attachment":[{"href":"https:\/\/protsci-us2b.univ-nantes.fr\/imutate\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=6"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}