Description
This tool combines point mutations obtained by site saturation mutagenesis. It performs permutations with the submitted positions and mutations. A new sequence is generated for each combination.
Three different modes for the treatment of mutations & positions are proposed:
| Mode | Operation | Input example |
| Default saturation mode | permutations with 19 amino | 3 |
| Exclusive saturation mode | permutations with 19 amino-acid minus n selected | 3,A- |
| Inclusion saturation mode | permutations only n selected amino-acid | 3,A+ |
Examples
- To do permutations with all the 20 amino-acids at positions 3 and 4:
- type 3 and 4 on separate lines in the mutation position text box
- To do permutations with all the amino-acids excluding A at position 3:
- type 3,A- in the mutation position text box
- To do permutations with only A at position 3:
- type 3,A+ in the mutation position text box
- To do permutations with all the amino-acids excluding A, E, F at position 3:
- type 3,A-E-F in the mutation position text box
- To do permutations with only A, E, F at position 3:
- type 3,A+E+F in the mutation position text box
- To do permutations with all the amino-acids excluding A, E, F at position 3 & all amino acids in position 4 & only A, E, F in position 5:
- type the following 3 lines in the mutation position text box
- 3,A-E-F
- 4
- 5,A+E+F
. Do not add a “minus” sign after the letter F.
. Do not add a “plus” sign after the letter F.