iMutate chimera

Gene shuffling mutagenesis

This script splits up two parent protein sequences into a given number of fragments. Next it shuffles the fragments between the two parent proteins. The tool keeps the fragments in the same order as in the parents.

Example

In order to undertand the tool, see the following example:

  • Parent protein A (green) that will be cut before positions 120 and 380 for generating three fragments
  • Parent protein B (blue), that will be cut before positions 120 and 370 for generating three fragments

chimera

Positions for cuts for protein 1 (one per line)
Positions for cuts for protein 2 (one per line)
or upload cut positions for protein 1
or upload cut positions for protein 2
Write or upload your sequence 1
Write or upload your sequence 2